Alachua Florida Commercial Properties for Sale

1,936 SF retail storefront in the heart of Downtown Alachua. The front features an open retail/showroom area with large storefront windows, while the rear includes additional space with a kitchen area, full shower, outdoor patio, and rear parking access. The zoning allows for a live/work business to occupy the space. Covered outdoor patio, rear access, and on-site parking create a unique opportunity in the downtown area. The building is also available to lease see GC539923 for more information.

14827 Main Street, Alachua

Listing courtesy of COLDWELL BANKER M.M. PARRISH REALTORS. 352-335-4999

$299,000

GC541531 MLS

Virtually Staged. PRICE SLASHED ANOTHER $50,000- FOR A TOTAL OF $75,000.00 IN PRICE REDUCTIONS. THIS IS A VERY MOTIVATED SELLER! Rare stand alone office building on almost a half acre lot on Main St in Downtown Alachua. 6 individual office rooms, conference room, computer/copier room reception/waiting room and microwave station. Building is in great condition with new roof put on in April 2026. Monument sign out front. Can be used for office, retail and numerous other uses. Originally it was a doctor's office, then a business incubator office, then a Real Estate office and most currently it has been an attorney's office.

14721 Main Street, Alachua

Listing courtesy of ALACHUA REALTY, LLC. 386-462-5805

$750,000

GC539705 MLS

Positioned for success, this highly visible .51-acre commercial property along Hwy 441 in Alachua presents a rare opportunity for investors, developers, or owner-users looking to capitalize on a prime location with exceptional exposure. Currently operating as an auto body repair shop, the property offers immediate functionality with the added advantage of strong redevelopment potential. Surrounded by a thriving mix of national and regional retailers, this location benefits from consistent traffic flow and outstanding accessibility. Its proximity to Progress Park, Santa Fe High School, and San Felasco Tech City places it in the path of continued growth and economic activity—making it an ideal setting for a wide range of commercial uses. The site is high and dry, offering peace of mind and ease of development, while the flexible zoning potential opens the door to numerous possibilities. Whether you’re envisioning retail, service-based business, or a reimagined commercial concept, this property provides the foundation to bring your vision to life. With strong visibility, high traffic counts, and established surrounding infrastructure, this location delivers the exposure every business needs to thrive. Opportunities like this—combining location, flexibility, and growth potential—are increasingly rare. Seize the chance to secure your place along one of Alachua’s most traveled corridors and position your business or investment for long-term success.

16260 Nw Us Highway 441, Alachua

Listing courtesy of HORIZON REALTY OF ALACHUA INC. 386-462-4020

$2,750,000

OM707990 MLS

Exceptional opportunity to own over 14,000 sq ft of commercial space in a prime location near historic downtown Alachua!!! Currently configured as a gym and medical office, this property offers a versatile layout featuring a large open central area and multiple private enclosed rooms, ideal for a variety of uses. The flexible floor plan can accommodate medical or professional offices, fitness or wellness centers, classrooms, retail showrooms, therapy suites, or co-working spaces. Located in a high-visibility area, this property benefits from close proximity to downtown amenities, restaurants, and shops. With its spacious design, excellent accessibility, and ample parking, this building provides endless potential for investors or business owners.

15043 Main Street, Alachua

Listing courtesy of THE AMERICAN DREAM. 180-045-1160

$1,200,000

GC530646 MLS

This 16.81-acre property offers a prime opportunity for mixed-use development, boasting approximately 700 feet of frontage along Highway 441 near the entrance to Turkey Creek, a well-established residential community with over 1,100 single-family and multi-family homes. The site features split zoning, providing flexibility for a wide range of uses. The front 8.5 acres are zoned Community Commercial (CC), allowing for high-demand uses such as gas stations, auto services, large-scale retail, hotels, medical and dental clinics, veterinary offices, pharmacies, and convenience stores. The rear 8.31 acres are zoned Agricultural (A) with a future land use designation of Residential Moderate Density, making it well-suited for residential development. Located in a high-visibility, rapidly growing corridor, this property is ideal for a thoughtfully planned mixed-use project that meets both commercial and residential demand. In the interim, benefit from steady income generated by five occupied rental units on the property, which include two mobile homes, one single-family residence, and one duplex.

12100 Nw Us Highway 441, Alachua

Listing courtesy of RESOURCE REALTY, LLC. 352-226-3201

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Alachua Florida Commercial Properties

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